POLLEN VIABILITY OF Eragrostis plana GENOTYPES FROM DIFFERENT GEOGRAPHIC POPULATIONS IN RIO GRANDE DO SUL
Palavras-chave:Acetic orcein 2%, Alexander reactive, pasture invaders, pollen viability, reproductive characteristics
Livestock production in southern Brazil is largely sustained by the production
of natural pastures, whose distinct botanical compositions, adapted to different
climatic and soil nuances, have allowed combining animal production and
environmental preservation over time. Among the main problems related to
vegetation management, is the disturbing expansion of the exotic grass Eragrostis
plana Ness., which deserves to be highlighted due to its high capacity for
colonization of the grasslands and the trend of excluding the native plant community.
Despite the importance of this species for the region, information on its biology are
insufficient, which impedes the establishment of efficient mechanisms for invasion
control. In this paper, the estimation of pollen viability of E. plana was studied by
staining, using acetic orcein 2% and Alexander reactive. Three different populations
were used, evaluating 6 genotypes per population. The young inflorescences were
collected and fixed in ethanol: acetic acid (3:1) for 24 hours room temperature, then
stored in 70% ethanol under refrigeration. To evaluate pollen viability, then 300
pollen grains of each blade were analyzed. All studied genotypes showed high pollen
viability for both stains, and the Alexander reactive proved to be more appropriate to
estimate the pollen viability of E. plana.
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